Intended Use:

Test for estimation of Urea in serum / plasma using UREASE BERTHELOT method.


Urea is the end product of protein metabolism. It is synthesized in liver and transported to kidney for excretion. Increased Urea / Urea Nitrogen levels are associated with renal diseases as well as dehydration, gastrointestinal hemorrhage and diabetic coma. Decreased values are observed in some cases of severe liver diseases. Urea can be estimated in serum / plasma using Urease / Chromogen method.

Urea Berthelot Test Procedure

Urea Berthelot Programming Video

Possible Errors in Urea Berthelot Test and Solutions


  • Enzymatic Method Specific For Urea
  • Liquid Stable Reagents With Long Shelf Life
  • Stable Reagent Blank
  • Fast Result
  • High Linearity
  • Stable Standard Factor


Methodology Urease – Berthelot
Sample Type Human Serum or Plasma
Number Of Reagents Two Liquid Reagents
Sample Volume Required Per Test 10 µL
Test Time Required 10 Minutes
Linearity 350 mg/dL
Automation Enabled
Pack Sizes 2X25 ML
4X50 ML
Kit components Reagent bottles.
Enzyme vial.
Standard vial.
Kit insert
Storage Temperature 2-8 0C
Shelf life 24 months
Kit dimensions 2X25 ML – 85x105x45 mm
4X50 ML – 105x170x45 mm
Approx. weight of kit 2X25 ML – 92 gms
4X50 ML – 268 gms
Production Capacity 600 mn ml per annum