Fouchet’s reagent

FOUCHET’S REAGENT

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Intended Use:

Fouchet’s reagent is used to detect Bilirubin (Bile) in urine.

Principle

For testing bilirubin in urine, two types of tests are available namely (A) Oxidation test-where bilirubin is oxidised to green biliverdin and (B) Diazotization test-where bilirubin is diazotised to a highly coloured compound. Fouchet’s reagent used in Fouchet’s test comes under oxidizing reagent or test. Barium chloride precipitates the sulphate radicals present in urine to form precipitate of barium sulphate. If bile pigments are present in urine, they adhere to these molecules. Ferric chloride present in Fouchet’s reagent then oxidises yellow bilirubin, in the presence of trichloroacetic acid to green biliverdin. Therefore, the development of green colour due to the formation of biliverdin indicates the presence of bilirubin(bile) in urine.

 

Fouchet's Reagent

Pack Sizes 1 x 100ml
1 x 500 ml
Kit components Reagent bottles
Kit insert.
Physical Appearance: Clear pale yellow liquid without any particles..
Signs of Deterioration: 1. Colour change
2. Growth
Kit dimensions 1 X100ml – 48x120x48mm
1 x 500ml – 100x200X 100mm
Approx. weight of kit 1 x 100 ml – 130 gms
1 x 500ml – 620 gms
Storage Temperature Room Temperature
Shelf life 60 months
Production Capacity 600 mn ml per annum