BRUCELLA A+M

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Intended Use:

Test for qualitative determination of antibodies against Brucella pathogen in human sera.

Introduction:

Human Brucellosis (Diurnal or undulant fever) is a common febrile illness caused by infection with bacteria of some of the Brucella species (Abortus, Melitensis). This undulant fever is associated with symptoms, which are often variable and non-specific with chills, fever, sweats and anorexia. On exposure the body responds to this antigenic stimulation by producing specific antibodies whose titres rise slowly at early stages and then increases. Specific antibodies to the Brucella species are detectable a few weeks after exposure and are of considerable importance in the diagnosis of Brucellosis. Information regarding the titre of antibodies can be obtained by using specific antigen suspensions.

Brucella A+M

Method Principle:

The smooth, attenuated stained antigen suspensions are mixed with the patient’s serum. Specific antibodies to Brucella antigens if present in the patient serum will react with the antigen suspension to produce an agglutination reaction. No agglutination indicates the absence of specific antibodies to Brucella antigens.

Kit Components

Brucella Abortus Antigen – 1 bottle of 5 ml.
Brucella Melitensis Antigen – 1 bottle of 5 ml.
Instructions for Use.

Specification

Procedure Qualitative only (Slide Test), Semi-quatitative method (Slide Test) as well as Quantitative method (Tube Test).
Procedur Time 1 min for qualitative and semi-quantitative method.
Sample Type Serum Only
Sensitivity Upto 1:320 dilution in Semi-quantitative and Quantitative test method.
Pack Sizes 2×5 ml
Storage Temperature 2- 80C
Shelf life 24 months
Kit dimensions 2×5 ml – 85x105x45 mm.
Approx. weight of kit 2×5 ml – 77 gms.
Production Capacity 600 mn ml per annum