Intended Use:

Test for estimation of ALT SGPT activity in serum / plasma using UV –Kinetic method.


Elevated serum SGPT (ALT) levels are found in hepatitis, cirrhosis, and obstructive jaundice. Levels of SGPT are only slightly elevated in patients following a myocardial infarction. Characteristically SGPT is generally higher than SGOT in acute viral or toxic hepatitis whereas for most patients with chronic hepatic disease SGPT levels are generally lower than SGOT levels. ALT (SGPT) is a reagent set for determination of SGPT enzyme activity in human serum / plasma based on UV Kinetic method, recommended by International Federation of Clinical Chemistry (IFCC).

Alt (SGPT) Test Procedure

Alt (SGPT) Programming Video

Alt(SGPT) Test Possible Errors


  • Method Specific To SGOT Using Aspartate Substrate
  • Liquid Stable Reagents With Long Shelf Life
  • Fast Result
  • High Linearity
  • Suitable For Fully Automated Chemistry Analyzer


Methodology Kinetic (IFCC recommended)
Sample Type Human Serum or Plasma
Reaction Type Kinetic
Number Of Reagents Two
Primary Wavelength 340 nm
Secondary Wavelength NA
Flow Cell Temperature 370C
Blank Settings Reagent
Blank Absorbance Limit > 0.900
Reagent Volume Required Per Test 1000 µl
Sample Volume Required Per Test 100 µL
Incubation Temperature 370C
Standard Available in the Kit No
Standard Concentration NA
Factor 1746
Linearity 1700 IU/L
Automation Enabled
Pack Sizes 50 ml – 1×40 ml + 1×10 ml
100 ml – 2×40 ml + 2×10 ml
Kit components Separate reagent bottles each for R1 and R2
Kit insert
Storage Temperature 2-8 0C
Shelf life 18 months
Kit dimensions 50 ml – 85x105x45 mm
100 ml -105x55x145 mm
Approx. weight of kit 50 ml – 88 gms
100 ml – 176 gms
Production Capacity 600 mn ml per annum