ALT (SGPT)
Intended Use:
Test for estimation of ALT SGPT activity in serum / plasma using UV –Kinetic method.
Introduction:
Elevated serum SGPT (ALT) levels are found in hepatitis, cirrhosis, and obstructive jaundice. Levels of SGPT are only slightly elevated in patients following a myocardial infarction. Characteristically SGPT is generally higher than SGOT in acute viral or toxic hepatitis whereas for most patients with chronic hepatic disease SGPT levels are generally lower than SGOT levels. ALT (SGPT) is a reagent set for determination of SGPT enzyme activity in human serum / plasma based on UV Kinetic method, recommended by International Federation of Clinical Chemistry (IFCC).
Alt (SGPT) Test Procedure
Alt (SGPT) Programming Video
Alt(SGPT) Test Possible Errors
Features:
- Method Specific To SGOT Using Aspartate Substrate
- Liquid Stable Reagents With Long Shelf Life
- Fast Result
- High Linearity
- Suitable For Fully Automated Chemistry Analyzer
Specification
| Methodology | Kinetic (IFCC recommended) |
| Sample Type | Human Serum or Plasma |
| Reaction Type | Kinetic |
| Number Of Reagents | Two |
| Primary Wavelength | 340 nm |
| Secondary Wavelength | NA |
| Flow Cell Temperature | 370C |
| Blank Settings | Reagent |
| Blank Absorbance Limit | > 0.900 |
| Reagent Volume Required Per Test | 1000 µl |
| Sample Volume Required Per Test | 100 µL |
| Incubation Temperature | 370C |
| Standard Available in the Kit | No |
| Standard Concentration | NA |
| Factor | 1746 |
| Linearity | 1700 IU/L |
| Automation | Enabled |
| Pack Sizes | 50 ml – 1×40 ml + 1×10 ml 100 ml – 2×40 ml + 2×10 ml |
| Kit components | Separate reagent bottles each for R1 and R2 Kit insert |
| Storage Temperature | 2-8 0C |
| Shelf life | 18 months |
| Kit dimensions | 50 ml – 85x105x45 mm 100 ml -105x55x145 mm |
| Approx. weight of kit | 50 ml – 88 gms 100 ml – 176 gms |
| Production Capacity | 600 mn ml per annum |