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Intended Use:

Test for estimation of Acid phosphatase activity in serum / plasma using α-Naphathylphosphate Kinetic method.


Increased activities of total and prostate specific acid phosphatase are associated with prostate gland disorders (cancer). Elevations in total enzyme activity are observed also in some liver diseases. It is a reagent set for quantitative determination of Acid phosphate activity based on kinetic method using naphthylphosphate.

Acid Phosphatase

Acid Phosphatase Errors Video

Acid Phosphatase Programming Video

Features Of Acid Phosphatase:

  • Kinetic Method Using α-Naphthyl Phosphate Substrate
  • Working Reagent Stable for One Week
  • Freedom to Test Total or Nonprostatic Acid Phosphatase
  • Fast Result
  • High Linearity
  • Two Reagents with Easy Preparation


Methodology Kinetic (α- Napthylphosphate)
Reaction Type Kinetic
Reaction Direction Increasing
Sample Type Human Serum or Plasma
Number of Reagents Single After Reconstitution
R1:R2 Mixing Ratio NA
Primary Wavelength 405 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
Blank Settings Distilled Water
Blank Absorbance Limit < 0.500
Reagent Volume Required Per Test 1000 µl
Sample Volume Required Per Test 100 µL
Delay Time 300 seconds
Read Time 60 seconds
Incubation Temperature 37 0C
Standard Available in the Kit NO
Standard Concentration NA
Factor 743
Linearity 75 IU/L
Automation Enabled
Pack Sizes 12 x 1 ml
1 x 50 ml
Kit components 1 bottle each for NAP and TAP Diluent.
Substrate vial.
Instructions for Use.
Storage Temperature 2-8 0C
Shelf life 24 months
Kit dimensions 12 ml – 85x105x45 mm
50 ml – 85x105x45 mm
Approx. weight of kit 12 ml – 66 gms
50 ml – 99 gms
Production Capacity 600 mn ml per annum