Test for estimation of antibodies against Streptolysin ‘’O ‘’ by using Latex turbidmertric method.
Streptolysin “O” is a toxic immunogenic exoenzyme produced by haemolytic Streptococci of groups A, C and G. Measuring the ASO antibodies are useful for the diagnostic of rheumatoid fever, acute glomerulonephritis and streptococcal infection. Rheumatic fever is an inflammatory disease affecting connective tissue from several parts of human body as skin, heart, joints etc. and acute glomerulonephritis is a renal infection that affects mainly to renal glomerulus.
The ASO-Turbilatex is a quantitative turbidimetric test for the detection of anti-steptolysin “O” (ASO) antibodies in human serum/plasma. Latex particles coated with steptolysin “O” are agglutinated when mixed with samples containing ASO. The agglutination causes an absorbance change, dependent upon the ASO contents of the patient sample that can be quantified by comparison from a calibrator of known ASO concentration.
|Sample Type||Human Serum or Plasma|
|R1:R2 Mixing Ratio||4:1|
|Primary Wavelength||540 nm|
|Blank Settings||Distilled Water|
|Blank Absorbance Limit||<0.300|
|Reagent Volume Required Per Test||1000 µl|
|Sample Volume Required Per Test||10 µL|
|Delay Time||10 seconds|
|Calibrator Available in the Kit||Yes|
|Pack Sizes||1×50 ml|
|Kit components||Reagent bottles – R1 and R2.
1 Calibrator vial.
Instructions for Use.
|Storage Temperature||2- 80C|
|Shelf life||24 months|
|Kit dimensions||1×50 ml – 85x105x45 mm|
|Approx. weight of kit||1×50 ml – 130 gms|
|Production Capacity||600 mn ml per annum|