Home / Our Products / Clinical Chemistry / HDL Cholesterol Direct

Intended Use:

Test for estimation of HDL- Cholesterol in serum / plasma using CHOD-POD method.


HDL particles regulate the cholesterol levels by uptaking and transport from peripheral tissues to liver. Low HDL cholesterol are associated with coronary heart diseases. It can be estimated in serum / plasma using special polymer detergent method.

HDL Cholesterol Direct Test Procedure

HDL Cholesterol Direct Programming Video

Possible Errors in HDL Cholesterol Direct Test and Solutions


  • It Is Specific Detergents Employed
  • Liquid Stable Reagents With Long Shelf Life
  • Suitable For Fully Automated Chemistry Analyzer
  • Fast Result
  • High Linearity


Methodology Special Polymer Detergent (CHOD-POD)
Reaction Type End Point
Sample Type Human Serum or Plasma
Number Of Reagents Two
R1:R2 Mixing Ratio 3:1
Primary Wavelength 546 nm
Secondary Wavelength 630 nm
Flow Cell Temperature 37 0C
Blank Settings Distilled Water
Blank Absorbance Limit <0.300
Reagent Volume Required Per Test 800 µl (600 µl R1 + 200 µl R2)
Sample Volume Required Per Test 5 µL
Incubation Time 10 Minutes
Incubation Temperature 37 0C
Standard Available in the Kit Yes
Standard Concentration On calibrator vial
Factor NA
Linearity 400 mg/dL
Automation Enabled
Pack Sizes 1×15 ml + 1×5 ml
2×30 ml + 2×10 ml
Kit components Reagent bottles.
Calibrator vial.
Instructions for Use.
Storage Temperature 2-8 0C
Shelf life 18 months
Kit dimensions 1×15 ml + 1×5 ml – 85X105X45
2×30 ml + 2×10 ml – 85X105X45
Approx. weight of kit 1×15 ml + 1×5 ml – 50 gms
2×30 ml + 2×10 ml -92 gms
Production Capacity 600 mn ml per annum