Intended Use:

Test for estimation of HDL Cholesterol in serum / plasma using Phosphotungstic Acid  method.

HDL PPT Test Procedure

HDL PPT Programming Video


  • Enzymatic Specific To Cholesterol (Free & Esterified)
  • Liquid Stable Reagent With Long Shelf Life
  • Fast Result
  • High Linearity
  • LCF (Lipid Clearing Factor) Incorporated For Lipamic Sample
  • Suitable for Fully Automated Chemistry Analyzer


Methodology Precipitating (Phosphotungstic Acid)
Reaction Type End Point
Sample Type Human Serum or Plasma
Number Of Reagents Single
R1:R2 Mixing Ratio NA
Primary Wavelength 546 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
Blank Settings Reagent
Blank Absorbance Limit <0.300
Reagent Volume Required Per Test 500 µl of Precipitating reagent and 1000 µl of Cholesterol
Sample Volume Required Per Test 500 µl serum and 50 µl supernatant
Incubation Temperature 37 0C
Standard Available in the Kit NO
Standard Concentration 50 mg/dL
Factor NA
Linearity 400 mg/dL
Automation Enabled
Pack Sizes 2×25 ml
4×50 ml
Kit components Reagent bottles.
Standard vial.
Kit insert.
Storage Temperature 2-8 0C
Shelf life 24 months
Kit dimensions 2×25 ml – 85x105x45 mm
4×50 ml – 105x170x 45 mm
Approx. weight of kit 2×25 ml – 92 gms
4×50 ml – 266 gms
Production Capacity 600 mn ml per annum