ALKALINE PHOSPHATASE
Intended Use:
Test for estimation of Alkaline Phosphatase activity in serum / plasma using PNPP method.
Introduction:
Serum Alkaline Phosphatase Test levels are of interest in the diagnosis of hepatobiliary disorders and bone disease associated with increased osteoblastic activity. Moderate elevations of Alkaline Phosphatase may be seen in Hodgkin’s disease, congestive heart failure, ulcerative colitis, regional enteritis, and intra-abdominal bacterial infections. Alkaline Phosphatase kitis a reagent set for determination ofAlkaline Phosphatase activity based on kinetic method using p-nitrophenylphosphate.
Alkaline Phosphatase Test Procedure
Alkaline Phosphatase Programming Video
Possible Errors in Alkaline phosphatase Test and Solutions
Features:
- Kinetic Method Using α-Naphthyl Phosphate Substrate
- Working Reagent Stable for One Week
- Freedom to Test Total orNonprostatic Acid Phosphatase
- Fast Result
- High Linearity
- Two Reagents with Easy Preparation
- Suitable for Fully Automated Chemistry Analyzer
Specification
| Methodology | Kinetic (PNPP Substrate) |
| Reaction Type | Kinetic |
| Reaction Direction | Increasing |
| Sample Type | Human Serum or Plasma |
| Number Of Reagents | Single After Reconstitution |
| R1:R2 Mixing Ratio | NA |
| Primary Wavelength | 405 nm |
| Secondary Wavelength | NA |
| Flow Cell Temperature | 37 0C |
| Blank Settings | Reagent |
| Blank Absorbance Limit | < 0.700 |
| Reagent Volume Required Per Test | 1000 µl |
| Sample Volume Required Per Test | 20 µL |
| Delay Time | 60 seconds |
| Read Time | 30 seconds |
| Incubation Temperature | 37 0C |
| Standard Available in the Kit | NO |
| Standard Concentration | NA |
| Factor | 2720 |
| Linearity | 2000 IU/L |
| Automation | Enabled |
| Pack Sizes | 12 x 1 ml 1 x 50 ml |
| Kit components | 1 bottle each for NAP and TAP Diluent. Substrate vial. Instructions for Use. |
| Storage Temperature | 2-8 0C |
| Shelf life | 24 months |
| Kit dimensions | 12 ml – 85x105x45 mm 50 ml – 85x105x45 mm |
| Approx. weight of kit | 12 ml – 66 gms 50 ml – 99 gms |
| Production Capacity | 600 mn ml per annum |