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Intended Use:

Test for estimation of Alkaline Phosphatase activity in serum / plasma using PNPP method.


Serum Alkaline Phosphatase Test levels are of interest in the diagnosis of hepatobiliary disorders and bone disease associated with increased osteoblastic activity. Moderate elevations of Alkaline Phosphatase may be seen in Hodgkin’s disease, congestive heart failure, ulcerative colitis, regional enteritis, and intra-abdominal bacterial infections. Alkaline Phosphatase kitis a reagent set for determination ofAlkaline Phosphatase activity based on kinetic method using p-nitrophenylphosphate.

Alkaline Phosphatase Test Procedure

Alkaline Phosphatase Programming Video

Possible Errors in Alkaline phosphatase Test and Solutions


  • Kinetic Method Using α-Naphthyl Phosphate Substrate
  • Working Reagent Stable for One Week
  • Freedom to Test Total orNonprostatic Acid Phosphatase
  • Fast Result
  • High Linearity
  • Two Reagents with Easy Preparation
  • Suitable for Fully Automated Chemistry Analyzer


Methodology Kinetic (PNPP Substrate)
Reaction Type Kinetic
Reaction Direction Increasing
Sample Type Human Serum or Plasma
Number Of Reagents Single After Reconstitution
R1:R2 Mixing Ratio NA
Primary Wavelength 405 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
Blank Settings Reagent
Blank Absorbance Limit < 0.700
Reagent Volume Required Per Test 1000 µl
Sample Volume Required Per Test 20 µL
Delay Time 60 seconds
Read Time 30 seconds
Incubation Temperature 37 0C
Standard Available in the Kit NO
Standard Concentration NA
Factor 2720
Linearity 2000 IU/L
Automation Enabled
Pack Sizes 12 x 1 ml
1 x 50 ml
Kit components 1 bottle each for NAP and TAP Diluent.
Substrate vial.
Instructions for Use.
Storage Temperature 2-8 0C
Shelf life 24 months
Kit dimensions 12 ml – 85x105x45 mm
50 ml – 85x105x45 mm
Approx. weight of kit 12 ml – 66 gms
50 ml – 99 gms
Production Capacity 600 mn ml per annum