BILIRUBIN TOTAL & DIRECT

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Bilirubin Total Test Procedure

Bilirubin Direct Test Procedure

Bilirubin Total Programming Video

Bilirubin Direct Programming Video

Intended Use:

Test for estimation of Bilirubin in serum / plasma using DMSO method.

Introduction:

Bilirubin is a yellow pigment in everyone’s blood and stool. Sometimes the liver can’t process the bilirubin in the body. This can be due to an excess of bilirubin, an obstruction, or inflammation of the liver. Direct bilirubin is elevated in obstructive jaundice and cirrhosis of liver whereas indirect bilirubin is elevated in prehepatic disorders (hemolysis of impaired conjugation or transport) in liver. Estimation of bilirubin can be done in serum / plasma using Jendrassik & Grof method.

Specification

Methodology Jendrassik & Grof
Sample Type Human Serum or Plasma
Reaction Type End Point
Number Of Reagents Double
Primary Wavelength 546 nm
Secondary Wavelength 600 nm
Flow Cell Temperature 370C
Blank Settings Distilled Water
Blank Absorbance Limit > 0.300
Reagent Volume Required Per Test 1000 µl
Sample Volume Required Per Test 20 µL
Incubation Temperature Room TemperatureC
Standard Available in the Kit No
Standard Concentration NA
Factor 15
Linearity 20 mg/dL
Automation Enabled

Specification

Pack Size 100 ml – 2×50 ml
1000 ml – 2X 500 ml
Mixing Ratio 19: 1
Kit components 1 bottle of T1 and 1 bottle of T2.
Kit insert
1 bottle of D1 and 1 bottle of D2.
Kit insert.
Storage Temperature 2-30 0C
Kit dimensions 2×50 ml – 85x105x45 mm
2×500 ml – 140x75x200 mm
Approx weight of kit 2×50 ml – 140 gms
2×500 ml – 1220 gms