BILIRUBIN TOTAL & DIRECT
Bilirubin Total Test Procedure
Bilirubin Direct Test Procedure
Bilirubin Total Programming Video
Bilirubin Direct Programming Video
Intended Use:
Test for estimation of Bilirubin in serum / plasma using DMSO method.
Introduction:
Bilirubin is a yellow pigment in everyone’s blood and stool. Sometimes the liver can’t process the bilirubin in the body. This can be due to an excess of bilirubin, an obstruction, or inflammation of the liver. Direct bilirubin is elevated in obstructive jaundice and cirrhosis of liver whereas indirect bilirubin is elevated in prehepatic disorders (hemolysis of impaired conjugation or transport) in liver. Estimation of bilirubin can be done in serum / plasma using Jendrassik & Grof method.
Specification
| Methodology | Jendrassik & Grof |
| Sample Type | Human Serum or Plasma |
| Reaction Type | End Point |
| Number Of Reagents | Double |
| Primary Wavelength | 546 nm |
| Secondary Wavelength | 600 nm |
| Flow Cell Temperature | 370C |
| Blank Settings | Distilled Water |
| Blank Absorbance Limit | > 0.300 |
| Reagent Volume Required Per Test | 1000 µl |
| Sample Volume Required Per Test | 20 µL |
| Incubation Temperature | Room TemperatureC |
| Standard Available in the Kit | No |
| Standard Concentration | NA |
| Factor | 15 |
| Linearity | 20 mg/dL |
| Automation | Enabled |
Specification
| Pack Size | 100 ml – 2×50 ml 1000 ml – 2X 500 ml |
| Mixing Ratio | 19: 1 |
| Kit components | 1 bottle of T1 and 1 bottle of T2. Kit insert 1 bottle of D1 and 1 bottle of D2. Kit insert. |
| Storage Temperature | 2-30 0C |
| Kit dimensions | 2×50 ml – 85x105x45 mm 2×500 ml – 140x75x200 mm |
| Approx weight of kit | 2×50 ml – 140 gms 2×500 ml – 1220 gms |