Intended Use:

Test for estimation of Creatinine in serum / plasma using Jaffé method.

Creatinine Test Procedure

Creatinine Programming Video

Possible Errors In Creatinine Test


  • Working reagent stability of 6 months thereby removing the need to prepare fresh working reagent daily.
  • Also available in single reagent ready-to-use format with a shelf life of 12 months eliminating the need to prepare working reagent at all.
  • Removes the negative interference of Bilirubin upto 20 mg/dl thereby increasing the dependability of the report even in high bilirubin samples such as neonatal samples.


Methodology Jaffe (Alkaline Picrate)
Reaction Type Rate Kinetic
Reaction Direction Increasing
Sample Type Human Serum or Plasma
Number Of Reagents Two
R1:R2 Mixing Ratio 1:1
Primary Wavelength 505 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
Blank Settings Reagent
Blank Absorbance Limit  <0.200
Reagent Volume Required Per Test 1000 µl
Sample Volume Required Per Test 50 µL
Delay Time 30 seconds
Read Time 60 seconds
Incubation Temperature 37 0C
Standard Available in the Kit Yes
Standard Concentration 2 mg/dL
Factor NA
Linearity 30 mg/dL
Automation Enabled
Pack Sizes 2×100 ml
2×500 ml
Kit components Reagent bottles – 1 bottle each for Picrate and Diluent.
Standard vial.
Instructions for Use
Storage Temperature 2-30 0C
Shelf life 24 months
Kit dimensions 2×100 ml – 105x55x145 mm
2×500 ml – 140x75x200 mm
Approx. weight of kit 2×100 ml – 266 gms
2×500 ml – 1220 gms
Production Capacity 600 mn ml per annum