Intended Use:

Test for qualitative detection troponin I in human whole blood, serum or plasma sample.

Introduction:

Troponin I (TnI) is part of troponin complex, which together with tropomyosin, forms the main component that regulates the Ca+2 –sensitive ATP-ase activity of actomyosin is striated muscle (skeletal and cardiac). The troponin complex consists of three subunit has a distinct function with TnC as the site of Ca+2 binding, TnT the tropomyosin binding, and Tnl as the inhibitory subunit. Different isoforms of Tnl exists in the skeletal and cardiac muscles (sTnl and cTnl, respectively) with distinct immunologic epitopes that allow the production of cardiac-specific Tnl antibodies. The cardiac marker, troponin I has been established as useful tools in the diagnosis of acute myocardial infraction (AMI). Troponin I is found in blood at elevated concentrations approximately 4-6 hours after the onset of chest pain and peak at 12-24 hours. Troponin I levels remain elevated for up to 14 days. The use if this marker is an aid in the diagnosis of AMI after myocardial function.