AST (SGOT)
Intended Use:
Test for estimation of SGOT activity in serum / plasma using UV –Kinetic method.
Introduction:
The activity of SGOT (AST) in serum is increased significantly during heart, liver, kidney and muscle disease. The activity of SGOT (AST) is increased in 4-8 hours following a myocardial infarction, reaching its peak in 2-3 days and declining on the fourth- or fifth-day post infarction. SGOT is a reagent set for determination of GOT (AST) in human serum / plasma based on UV Kinetic method, recommended by International Federation of Clinical Chemistry (IFCC).
Ast (SGOT) Test Procedure
Ast (SGOT) Programming Video
Ast(SGOT) Test Possible Errors
Features:
- Method Specific To SGOT Using Aspartate Substrate
- Liquid Stable Reagents With Long Shelf Life
- Fast Result
- High Linearity
- Suitable For Fully Automated Chemistry Analyzer
Specification
| Methodology | Kinetic (IFCC recommended) |
| Sample Type | Human Serum or Plasma |
| Reaction Type | Kinetic |
| Number Of Reagents | Two |
| Primary Wavelength | 340 nm |
| Secondary Wavelength | NA |
| Flow Cell Temperature | 370C |
| Blank Settings | Reagent |
| Blank Absorbance Limit | > 0.900 |
| Reagent Volume Required Per Test | 1000 µl |
| Sample Volume Required Per Test | 100 µL |
| Incubation Temperature | 370C |
| Standard Available in the Kit | No |
| Standard Concentration | NA |
| Factor | 1746 |
| Linearity | 1700 IU/L |
| Automation | Enabled |
| Pack Sizes | 50 ml – 1×40 ml + 1×10 ml 100 ml – 2×40 ml + 2×10 ml |
| Kit components | Separate reagent bottles each for R1 and R2 Kit insert |
| Storage Temperature | 2-8 0C |
| Shelf life | 18 months |
| Kit dimensions | 50 ml – 85x105x45 mm 100 ml -105x55x145 mm |
| Approx. weight of kit | 50 ml – 88 gms 100 ml – 176 gms |
| Production Capacity | 600 mn ml per annum |