ADA(Adenosine Deaminase)

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Intended Use:

Test for quantitative estimation of ADA activity in serum, plasma and other body fluids.

Introduction:

ADA is an enzyme catalysing the deamination reaction from adenosine to inosine. The enzyme is widely distributed in human tissues, and is especially high in T lymphocytes. Elevated serum ADA activity has been observed in patients with acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis, liver cirrhosis, viral hepatitis and hepatoma Increased ADA activity was also observed in patients with tuberculous effusions. Determination of ADA activity in patient serum may add unique values to the diagnosis of liver diseases in combination with ALT or γ-GT (GGT) tests. ADA assay may also be useful in the diagnosis of tuberculous pleuritis.

 

Features:

  • Enzymatic Method Specific for ADA
  • Liquid Stable Reagent With Long Shelf Life
  • Fast Result
  • High Linearity

Specification

Methodology PNP-XOD
Sample Type : Human Serum or Plasma or Body Fluids
Reaction Type Kinetic
Number Of Reagents Two
Primary Wavelength 546 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
Blank Settings Reagent
Blank Absorbance Limit Distilled Water
Reagent Volume Required Per Test 600 µl
Sample Volume Required Per Test 10 µL
R1:R2 Mixing Ratio 2:1
Incubation Temperature 37 0C
Calibrator Available in the Kit Yes
Calibrator Concentration On Vial
Factor NA
Linearity 200 IU/L
Automation Enabled
Linearity 75 IU/L
Pack Sizes 15 ml – 1×10 ml + 1×5 ml
30 ml – 1×20 ml + 1×10 ml
Kit components Reagent bottles
Calibrator vial – 1 ml liquid stable.
Instructions for Use
Storage Temperature 2-8 0C
Shelf life 18 months
Kit dimensions 15 ml – 85X105X45 (MM)
30 ml – 85X105X45 (MM)
Approx. weight of kit 15 ml – 45 gm
30 ml – 65 gm
Production Capacity 600 mn ml per annum